TRANSFER OF PYRIDOXAL 5'-PHOSPHATE FROM ALBUMIN-PYRIDOXAL 5'-PHOSPHATE COMPLEX TO APO-ASPARTATE AMINOTRANSFERASE
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چکیده
منابع مشابه
Transfer of pyridoxal 5'-phosphate from albumin-pyridoxal 5'-phosphate complex to apo-aspartate aminotransferase.
Pyridoxal 5•L-phosphate (PLP) is known to combine with bovine serum albumin to form a(1:1) complex which scarcely dissociates, even when subjected to intensive dialysis. When this complex was incubated with apo-aspartate aminotransferase (apoGOT) for an appropriate time and the preincubated mixture then submitted to the usual GOT assay, the appearance of GOT activity was obviously confirmed, in...
متن کاملBinding of Pyridoxal 5'-Phosphate
1. The a and ,B subforms of aspartate aminotransferase were purified from pig heart. 2. The a subform contained 2mol of pyridoxal 5'-phosphate. The apo-(a subform) could be fully reactived by combination with 2mol of cofactor. 3. The protein fluorescence of the apo(a subform) decreased non-linearly with increase in enzyme activity and concentration of bound cofactor. 4. It is concluded that the...
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1. Oestrone 3-glucuronide has been identified as the reaction product after incubation of oestrone with a rabbit-liver microsomal preparation, uridine diphosphate glucuronic acid being used as the glucuronic acid donor. The formation of this compound was studied under various experimental conditions. 2. The reaction showed a maximum at pH 8.08-2, and Km for oestrone was found to be 9-7 x 10-5M....
متن کاملBinding of pyridoxal 5-phosphate to cystathionase.
The binding of pyridoxal 5-phosphate to the apoprotein of the enzyme cystathionase from rat liver was investigated by two independent methods, absorption and fluorescence spectroscopy. The increase in absorbance at 525 nm associated with Schiff’s base formation was used to investigate the binding of pyridoxal-5-P at a protein concentration of 1 X 10e5 M. A model based on two classes of independ...
متن کاملPyridoxal 5'-phosphate modulates expression of cytosolic aspartate aminotransferase gene by inactivation of glucocorticoid receptor.
The level of mRNA for cytosolic aspartate aminotransferase (cAST) in the liver of vitamin B6-deficient rats was found to be 7-fold higher than that of the control rats. The administration of hydrocortisone to adrenalectomized vitamin B6-deficient rats induced expression of hepatic cAST mRNA and the induction was suppressed by the simultaneous administration of pyridoxine. Since the 5' regulator...
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ژورنال
عنوان ژورنال: Journal of Nutritional Science and Vitaminology
سال: 1973
ISSN: 0301-4800,1881-7742
DOI: 10.3177/jnsv.19.229